How does extracellular electron transfer (EET) compare in different strains (A2-165, HTF-217, HTF-238) of Faecalibacterium prausnitzii?

Kassai, Sonia (2020) How does extracellular electron transfer (EET) compare in different strains (A2-165, HTF-217, HTF-238) of Faecalibacterium prausnitzii? --Choose an option--, Health & Life Sciences.

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Abstract

One of the most abundant commensal microbes that can be found in the human gut is Faecalibacterium prausnitzii. Because F. prausnitzii is involved in the production of butyrate, which is essential in maintaining good gut health, it is important to maintain its high numbers. In inflammatory bowel disease patients are found to have decreased levels of F. prausnitzii. It has previously been discovered that when flavins are present, F. prausnitzii acts as an electron shuttle and shuttles electrons when exposed to oxygen. It has been found that F. prausnitzii can use riboflavin as a mediator for extracellular electron transfer. However, as F. prausnitzii does not produce riboflavin itself, it relies on an exogenous supply from the dietary intake of an individual. Previous studies also have shown that riboflavin mediates the oxidation of NADH. In regular conditions, F. prausnitzii relies on a Na+ driven process that catalyzes the transfer of electrons from NADH to ubiquinone. While under oxidative stress, the bacterium uses a Rnf complex to catalyze the transfer of electrons between NADH and ferredoxin. These are proposed to be two process of extracellular electron transfer. In this study, various strains of F. prausnitzii were compared as their potential for extracellular electron transport varies. The comparison was done looking at the genetic components for proteins related to both the Na+ driven and Rnf processes.

Type:	Thesis (--Choose an option--)
Major:	Health & Life Sciences
Supervisor:	Harmsen, H.J.M.
Datum van aanlevering:	08 Jul 2020 09:15
Last modified:	08 Jul 2020 09:15
URI:	http://ucg.studenttheses.ub.rug.nl/id/eprint/54

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